Applyo Jena
Applyo Jena
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RT-qPCR

RT-qPCR

RT-qPCR Beads for Single Reactions

incl. VAT plus Shipping Costs

Applyo RT-qPCR product for reverse transcriptase – quantitvative PCR analyses with target detection possible in 5-plex (e.g. detection with 488 nm, 533 nm, 552 nm, 581 nm, 652 nm, 678 nm wave length depending on the filter setup of your thermocycler).

268,80 € – 273,80 €

Description

For in vitro use and RUO only!

Applyo Beads
Shipping Room temperature
Storage conditions Store at 20°C. Short term storage at temperatures up to 30°C is also possible.
Shelf life 12 months
Form Lyophilized
Concentration Each 1x Appylo Bead contains all components for a 25 µl RT-qPCR-reaction
Description Applyo RT-qPCR Beads contain all reagents for detection of RNA (and DNA) in a lyophilized bead format. This includes among others a polymerase and reverse transcriptase and buffer components for performing a RT-qPCR reaction. User only needs to add target specific primers and probes.

Each Applyo Bead is designed for a single 25 µl RT-qPCR-reaction-well. The beads are pre-mixed, guaranteeing reliable amplification results with minimal pipetting effort. Our ready-to-use bead-format with instantly resuspending reaction mixes ensures time-saving fast experimental setup with low risk of cross-contamination during handling.
Product safety

Available Options

Cat. No.QuantityPrice 
12 Stripes à 8 Beads
268,80 €
96 Beads in Standard PCR Plate
268,80 €
96 Beads in Fortitude 1D PCR Plate
271,05 €
96 Beads in Fortitude 2D PCR Plate
273,80 €

Handling

Applyo RT-qPCR Beads

Applyo Beads are stable under ambient conditions. Exposure to high moisture (>80 % rH) for more than 30 minutes or placing beads on ice may result in moisture condensation and may impair the PCR-performance due to the reduced solubility of the Applyo Beads. Therefore, limit the exposure to humidity (e.g. in the air) to a minimum until the bead is reconstituted with the primer-probe mixture and carefully reseal the package with remaining Applyo Beads if only a part of the beads is applied. Resealed Applyo Beads should be used within 3 months.

Recommended RT-qPCR protocol for Applyo RT-qPCR Beads

Handling of Applyo Beads

Open the tube containing the Applyo Bead just right before addition of primer-probe mixture.
Solve the Applyo Bead directly in the tube that it is provided in.

Protocol at a glance

Component Stock conc. Volume in 25 µl assay Final conc.
Applyo Bead 1x 1 µl (bead volume will add 1 µl to total reaction volume) 1x
Primer 1 µl – 24 µl ~ 200 – 800 nM
Probes 1 µl – 24 µl ~ 50 nM – 200 nM
RNA sample 1 µl – 24 µl 20 – 100,000 copies / reaction
Nuclease-free water Fill up to 25 µl

Recommended cycling program for adjustment to your requirements:

Temperature* Time*
50°C 3 min.
95°C 1 min.
95°C 5 sec. Repeat 45x*
58°C 30 sec.
Signal readout

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