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Viral Detection

Viral Detection

SARS-CoV-2 RT-qPCR Beads (RUO) for Single Reactions

incl. VAT plus Shipping Costs

Applyo SARS-CoV-2 RT-qPCR for quantitvative RT-qPCR analysis of RdRP, N1 and E gene of SARS-CoV-2 in FAM, TXR and Cy-5 channel.

384,00 € – 389,00 €

Description

For in vitro use and RUO only!

Applyo Beads
Shipping Room temperature
Storage conditions Store at 20°C. Short term storage at temperatures up to 30°C is also possible.
Shelf life 12 months
Form Lyophilized
Concentration Each 1x Appylo Bead contains all components for a 25 µl SARS-CoV-2 RT-qPCR-reaction
Description Applyo SARS-CoV-2 RT-qPCR Beads contain all reagents for detection of SARS-CoV-2 RNA in a lyophilized bead format. This includes among others a Hot-Start Taq DNA Polymerase and reverse transcriptase as well as primers and probes for SARS-CoV-2 RNA detection.

Each Applyo Bead is designed for a single 25 µl RT-qPCR-reaction-well.
The beads are pre-mixed, guaranteeing reliable amplification results with minimal pipetting effort. Our ready-to-use bead-format with instantly resuspending reaction mixes ensures time-saving fast experimental setup with low risk of cross-contamination during handling.
Product safety

Available Options

variantsQuantityPrice 
12 Stripes à 8 Beads
384,00 €
96 Beads in Standard PCR Plate
384,00 €
96 Beads in Fortitude 1D PCR Plate
386,50 €
96 Beads in Fortitude 2D PCR Plate
389,00 €

Handling

Applyo SARS-CoV-2 RT-qPCR Beads

Applyo Beads are stable under ambient conditions. Exposure to high moisture (>80 % rH) for more than 30 minutes or placing beads on ice may result in moisture condensation and may impair the PCR-performance due to the reduced solubility of the Applyo Beads. Therefore, limit the exposure to humidity (e.g. in the air) to a minimum until the bead is reconstituted with the RNA target and carefully reseal the package with remaining Applyo Beads if only a part of the beads is applied. Resealed Applyo Beads should be used within 3 months.

Recommended RT-qPCR protocol for Applyo SARS-CoV-2 RT-qPCR Beads

Handling of the Applyo Beads

Open the tube containing the Applyo Bead just right before addition of RNA sample. Prepare RNA sample according to your protocol in PCR-clean water or 10 mM Tris pH 8.0-buffer. Solve the Applyo Bead directly in the tube that it is provided in.

Protocol at a glance

Component Stock conc. Volume in 25 µl assay Final conc.
Applyo Bead 1x 1 µl (bead volume will add 1 µl to total reaction volume) 1x
RNA sample 1 µl – 24 µl 20 – 100,000 copies / reaction
Nuclease-free water Fill up to 25 µl if required

Recommended cycling program:

Temperature* Time*
50°C 3 min.
95°C 1 min.
95°C 5 sec. Repeat 45x*
58°C 30 sec.
Signal readout